|
KMID : 0620920070390060828
|
|
Experimental & Molecular Medicine
2007 Volume.39 No. 6 p.828 ~ p.838
|
|
|
The centrosomal localization of KM-HN-1 (MGC33607) depends on the leucine zipper motif and the C-terminal coiled-coil domain
|
|
Park Hye-Jeong
Seo Hyun-Joo
Kim Hyun-Woo
Kim Jung-Soon
Hwang So-Yoon
Seong Yeon-Sun
|
|
|
Abstract
|
|
|
|
KM-HN-1 is a C-terminal coiled-coil domain containing protein previously referred to as image clone MGC33607. This protein has been previously identified as a cancer/testis antigen and reported as nuclear and chromatin localizing protein. We raised polyclonal antisera with the GST fusion protein and identified them as a 105 kDa protein. Motif analysis showed that this protein harbors the leucine zipper motif in internal 1/3 region and the coiled-coil domain in the C-terminal region. Using the full length and various deletion mutants, we determined the motif that governs the subcellular localization of KM-HN-1. Immunofluorescence staining of the endogenous KM-HN-1 and various kinds of GFP-tagged KM-HN-1 revealed that KM-HN-1 localizes to the centrosomes as well as nucleus. The centrosomal localization-determining region of this protein is C-terminal coiled-coil domain in which the leucine zipper motif and the nuclear export signal (NES) harbor
|
|
|
KEYWORD
|
|
|
CCDC110 protein, human, cell nucleus, centrosome, protein structure, tertiary, protein transport
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|